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Several environmental studies using the laccase gene were published so far. They are cover different research question, study sites, soil compartments, etc. The table here should help to identify studies of interest. The order is chronologically. References are listed under laccase project entry page.
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Study
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Site / forest type
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Compartments
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RNA/DNA
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Total seqs / samples
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Outcome
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Luis et al. 2004
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Germany, Beech-Oak
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O, A, B
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DNA
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~250 (64 types) of 5 cores
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method developed
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Luis et al. 2005a
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Germany, Beech-Oak
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Org. horizon
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RNA
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~120 (14 types) of 5 cores
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method developed
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Luis et al. 2005b
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Germany, Beech-Oak
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O, A, B
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DNA
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~600 (167 types) of 13 cores
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diversity effects horizontally and vertically, saprotrophs dominate the O, mycorrhizals the A&B horizon
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Blackwood et al. 2007
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USA, Oak/Sugar Maple/Basswood
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O
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DNA
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~60 (21 types); qPCR & LH-PCR, copy no. 0.5-8 Mio/g; 3 sites, 3 reps, 3 treat- ments, 2 seasonal samplings
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Hofmockel et al. 2007
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USA, Oak/Sugar Maple/Basswood
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O, A
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DNA
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qPCR & LH-PCR, copy no. 0.1-10 Mio/g; 3 sites, 3 reps, 3 treatments, 4 samplings
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Kellner et al. 2008
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Germany, Beech-Oak & Grassland
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O, A, B & Grassland upper layer
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DNA
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bacterial laccases; ~170 (143 types) of 6 cores
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high diversity of bacterial lmco-genes in forests found, function not clear
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Kellner et al. 2009
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Germany, Beech
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Org. horizon
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DNA&RNA
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>400 (73 DNA, 42 RNA types) of 18 cores (3 reps, 6 samplings)
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seasonal changes of lcc diversity and expression in beech forest
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Hassett et al. 2009
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USA, Michgan, Sugar Maple
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O, A
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DNA
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qPCR & LH-PCR, copy no. from 0.5-50 Mio/g; 4 sites (total 12 control, 12 N-plots)
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Lauber et al. 2009
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USA, Oak/Sugar Maple/Basswood
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O
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DNA
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>200; qPCR, copy no. 3-4 Mio/g; 3 sites 3 reps, 3 treatments
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Artz et al. 2009
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Australia, Eucalyptus forest
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upper soil layer
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DNA
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~200 sequences, 95 OTU; 18 sites: 6 control, 6 2yr burning, 6 4yr burning
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burning declines phenoloxidase activity, but increases basidiomycete laccase gene diversity and eveness
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Kellner et al. 2010
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USA, Michgan, Sugar Maple
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O
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RNA
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few expressed types, 1 field site, 3 control, 3 N-plots
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expressed laccases in control and in N-plots, besides many other enzymes
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Theuerl et al. 2010
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Germany, Spruce
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O (Oe,Oa), A, B
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DNA
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>200 (92 types), 3 treatments (plots), 3-5 to pool/plot, 2 seasonal samplings
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response to reduced N, also roof effects, no changes in enzymes and decomposition
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Ausec et al. 2011
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Slovenia, Fen & Bog soil
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upper 30cm
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DNA
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bacterial Laccases, 27-55 seqs per soil core, 3 fen, 3 bog cores, 241 total seqs
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1/3 lcc types probably from Acidobacteria; novel 2-domain laccases; high diversities
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Christ et al. 2011
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Germany, Beech & Spruce
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B, rocks
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DNA
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86 types in bulk soil, 39 types on stones, 8 sites sampled
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differences soil vs. stone, pH effects, weathering of stones unlikely
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Edwards et al. 2011
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USA, Michgan, Sugar Maple
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O
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RNA
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4 sites (3 controls, 3 N-plots) in 2009, one site 2007; qPCR
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downregulation of laccase under increased N deposition
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Chen et al. 2013
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China
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DNA&RNA
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